Fig. 2

Effects of NF-κB inhibition on over-mechanical stretching stress-induced p65 expression in rat nucleus pulposus cells. a Western blot analysis for the protein expression of p65, GAPDH was used as loading control. The relative expressions were normalized to control (b). c The nuclear translocation of p65 was measured by Fluorescence immunocytochemistry. d Real-time PCR was used to measure the mRNA expressions of p65. Data are presented as mean ± SD. ***p < 0.001 between the indicated groups