Fig. 3

PPARγ knockdown suppressed the effect of MAR1 on LPS-induced inflammatory response in RAW264.7 cells and hPBMCs. Cells were treated with LPS (10 µg/ mL) for 24 h or treated with 10 nM of MAR1 for 12 h before LPS treatment. Each experiment was repeated in triplicate. a, Efficiency of RNA interfering was assayed by RT-qPCR. b, The protein level of PPAR-γ was detected by Western blot in different cell groups; c, The mRNA levels of TNF-α, IL-1β and IL-6 were determined by RT-qPCR in different cell groups. d, TNF-α, IL-1β and IL-6 secretion were measured using ELISA. Data were shown as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001