Fig. 1

MAR1 inhibited the inflammatory response of RAW264.7 cells and hPBMCs induced by LPS in a dose-dependent manner. RAW264.7 cells and hPBMCs were pretreated with different concentrations (0.1 nM, 1 nM, 10 nM and 100 nM) of MAR1 for 12 h and then exposed to LPS (10 µg/mL) for 12 h. a, TNF-α, IL-1β and IL-6 expression were detected by RT-qPCR; b, TNF-α, IL-1β and IL-6 secretion were measured using ELISA. c, The protein levels of SIRT1, PGC-1α and PPAR-γ were evaluated by Western blot. Data were shown as the means ± SD. Each experiment was repeated in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001