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Fig. 4 | Journal of Inflammation

Fig. 4

From: Dendritic cells transduced with glioma-expressed antigen 2 recombinant adenovirus induces specific cytotoxic lymphocyte response and anti-tumor effect in mice

Fig. 4

Specific TNF-α by ELISPOT assay

GLEA2-specific cytotoxic T lymphocytes (CTLs) were generated in vitro by weekly stimulation of splenocytes with irradiated autologous DCs transduced with Ad-GLEA2. The single-cell suspensions of splenocytes were prepared as effector cells to quantify specific TNF-α releasing cells. Triplicate experiments showed consistent results. Compared with controls,*P < 0.05.

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