Specific TNF-α by ELISPOT assay
GLEA2-specific cytotoxic T lymphocytes (CTLs) were generated in vitro by weekly stimulation of splenocytes with irradiated autologous DCs transduced with Ad-GLEA2. The single-cell suspensions of splenocytes were prepared as effector cells to quantify specific TNF-α releasing cells. Triplicate experiments showed consistent results. Compared with controls,*P < 0.05.