Fig. 5

Immunohistochemical staining of COX-2 in the paw tissue. a Paw tissue sections were immunostained for COX-2 at 6 and 24 h in the saline, CRG, and CRG + US groups. Arrows indicate representative COX-2 positive cells. b The expression levels of COX-2 are quantified by image J. The graph shows mean ± S.E. of 3 independent experiments (n = 3). Statistical comparison was conducted on the saline versus CRG groups and the CRG versus CRG + US groups. *P < 0.05, ** P < 0.01, and *** P < 0.001. Scale bar: 100 μm