Fig. 2

Histological analysis of paw edema. a Tissue sections from the hind paw were stained with H&E for histopathologic examination at 6 and 24 h in the saline (control), carrageenan-injected (CRG) and LIUS-stimulated (CRG + US) groups. The up down arrow indicates the diffused dermal layer and the asterisk shows interstitial edema in the CRG and CRG + US groups. The polymorphonuclear leukocytes are presented at higher magnification in the dotted box (× 100). b Multiple sections were used to determine the relative area of dermal layers using ImageJ. Quantitative data are presented as mean ± S.E. of three paw sections each from individual rats (n = 3). *P < 0.05, ** P < 0.01, and *** P < 0.001 compared to the CRG group