Fig. 6

Phagocytosis of Apo-J cells by NK-4-treated THP-1 macrophages induces Akt activation. NK-4 (5 μM)-treated THP-1 macrophages were co-cultured for 1 h with Cell Tracker Red-stained Apo-J cells at a 1:1 ratio, followed by immunostaining for CD86 (a). The frequency of macrophages engaged in phagocytosis of Apo-J cells is shown (b). Phosphorylation of Akt (Ser473) in NK-4 (5 μM)-treated THP-1 macrophages incubated with or without Apo-J cells was determined by western blot. A representative blot is shown (c). The optical density ratio of phospho-Akt to total Akt is shown (d). Graphs show the mean ± SEM of triplicate cultures and are representative of two independent experiments with similar results