Fig. 6
From: MiRNA-494 enhances M1 macrophage polarization via Nrdp1 in ICH mice model
Nrdp1 interacted with transcription factor C/EBP-β. a NIH-3 T3 cells were transduced with pcDNA3.1-Nrdp1 and pcDNA3.1-C/EBP-β, and treated with or without IL-4 (10 ng/ml) for 1 h. Anti-Nrdp1 antibody could pull down C/EBP-β from NIH-3 T3 cell lysates and anti-C/EBP-β antibody could also pull down Nrdp1 from NIH-3 T3 cell lysates in the immunoprecipitant. b NIH-3 T3 cells were transduced with pcDNA3.1-Nrdp1 and pcDNA3.1-C/EBP-β, and treated with or without IL-4 (10 ng/ml) for 1 h. The interaction between Nrdp1 and C/EBP-β could be enhanced by IL-4 stimulation. c Macrophages were stimulated with 10 ng/ml IL-4 for 1 h. Cell lysates were immunoprecipitated with anti-C/EBP-β antibody. IL-4 stimulation induced the ubiquitination of C/EBP-β, and upregulation of Nrdp1 promoted ubiquitination of C/EBP-β