Fig. 3

NGF suppressed the production of proinflammatory cytokines in HCECs induced by TLR3 activation. a Increased secretion of IL-8 and IL-6 in HCECs stimulated with poly (I:C) for 24 h in a dose-dependent manner. b-c Various concentrations of NGF could suppress the secretion of IL-6 and IL-8 in HCECs treated with 25 μg/ml poly (I:C) for 24 h. d TrkA phosphorylation was strongly increased after coincubation with NGF (50 ng/ml) in response to poly (I:C) (25 μg/ml) stimulation for 24 h. e TLR3 phosphorylation was decreased after coincubation with NGF (50 ng/ml) in response to poly (I:C) (25 μg/ml) stimulation for 1 h. f Reciprocally, TLR3 activation decreased intracellular NGF protein levels in HCECs treated with poly (I:C) for 24 h. g Stimulation with poly (I:C) for 24 h decreased NGF secretion by HCECs in a dose-dependent manner. Data represented mean ± SD from three independent representative experiments in each condition