Fig. 5
From: The role of N-glycosylation of CD200-CD200R1 interaction in classical microglial activation
N44Q mutant impaired CD200-CD200R1 interaction. Representative flow cytometric analysis of receptor density on the cell surface. Various amounts of biotinylated CD200 (10–40 μL) were added to 105 BV2 cells that were transiently transfected with empty vector, WT, or N44Q mutant. The numbers of biotinylated CD200R-bound CD200 were quantified using biotinylated CD200. Data are presented as means ± SEM (* p < 0.05, WT vs control; +p < 0.05, N44Q vs WT)