Fig. 1

The increased expression of TRPA1 in the IL-1β-treated rat chondrocytes. a Rat chondrocytes were stimulated with IL-1β (0–10 ng/ml) for 12 or 24 h, and thereafter total TRPA1 mRNA were measured by PCR. b Chondrocytes cultures were stimulated with IL-1β (0, 5, 10 ng/ml) for 12 and 24 h, respectively, and then TRPA1 protein expression was detected with Western blot. The PCR experiment were carried out in duplicate and the quantitation of protein expression was done by densitometry analysis of the bands from three independent measurements. Values were normalized against GAPDH, and analyzed with ImageJ software. Results are expressed as mean ± SEM. ^*p < 0.05, ^**p < 0.01. TRPA1 transient receptor potential ankyrin 1, IL interleukin