Fig. 4

Negative correlation between ZNF580 and IL-6 after dose-dependent LPS-stimulation. MonoMac6 cells were stimulated with different LPS concentrations ranging from 0.1 to 10 ng/mL for 24 H. IL-6 mRNA (a), quantified by real-time-PCR and normalised to corresponding GAPDH-values, and protein in the supernatant (b), measured by ELISA, were dose-dependently increased (shown as mean ± SEM, n = 3). ZNF580 protein was analysed by immunoblotting (c). IL-6 secretion was measured over 24 h (b and d). ZNF580 was determined at the end of these 24 h and normalised to β-actin values (d). Under these conditions IL-6 and ZNF580 were inversely correlated (d). For each of four different concentrations of LPS three different samples were analysed and four untreated samples were analysed