Fig. 5

Effect of medium glutamine on IL-6 response to LPS and MSO. (a) Peritoneal macrophages were prepared as previously described but were incubated in medium glutamine concentrations ranging from 0 to 4 mM one hour prior to the addition of LPS. As indicated, cells were also treated with 9 mM MSO. At 6 h after LPS treatment, IL-6 in the culture medium was assayed by ELISA. (s ± SEM, n = 6) (b) Peritoneal macrophages were treated with either 1, 4.5, or 9 mM MSO in medium lacking glutamine for 1 h prior to a 6-h exposure to LPS. Control values were normalized to 100%, and average values from treated samples were plotted in comparison to those controls. All 3 of the tested MSO concentrations yielded significant reductions in medium IL-6, with no significant difference between the % reduction of IL-6 between any of the concentrations