Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease

Table 2 Primers designs used for Real Time Polymerase Chain Reaction (RT-PCR)

GENE	GENEBANK	OLIGO LEFT	OLIGO RIGHT	UNIVERSAL PROBE LIBRARY	AMPLICON SIZE
CARD9	NM_052814.3, NM_052813.4	ggagacgctcgtcctgag	aggtcctgggggagtgag	79	gagacgctcgtcctgagctccgacctggaagatggctcacccaggaggtcccaggagctctcactcccccaggacct
CARD10	NM_014550.3	actaccccgaacacttcacg	gtcatcaagaattgggtcagg	72	actaccccgaacacttcacgctgctcacgggccaggaacccgcccagcgctgctccatgatcctcgatgaggaggggcctgagggcctgacccaattcttgatgac
CARD11	NM_032415.4	cagaggagctgcgagacaa	ggtgcttgtacatttcacagtcc	1	cagaggagctgcgagacaagtacctggaggagaaggaggacctggagctcaagtgctcgaccctgggaaaggactgtgaaatgtacaagcacc
CARD14	NM_024110.3	gagctcctagacacggcaga	cgagacatcaagccttccag	71	gagctcctagacacggcagaccttccgcagctggaaagcagcctgcagccagtctcccctggaaggcttgatgtctc
CARD15	NM_022162.1	gtgtcctcctcggacattct	ggaccctgagaccagcag	36	gtgtcctcctcggacattctccgggttgtgaaatgtgctcgcaggaggcttttcaggcacagaggagccagctggtcgagctgctggtctcagggtcc
GAPDH	NM_002046.3	agccacatcgctcagaca	gcccaatacgaccaaatcc	60	agccacatcgctcagacaccatggggaaggtgaaggtcggagtcaacggatttggtcgtattgggc

CARD9 assay was designed to detect both transcript isoforms (Universal Probe Library)

ISSN: 1476-9255