Fig. 4

NF-κB p65, c-Jun and CEBPδ are involved in HDAC2 regulation of LPS-induced inflammation. RAW264.7 cells were transfected with siRNA targeting HDAC2 for 48 h, and then stimulated with 100 ng/ml LPS for 2 h. HDAC-2 knockdown (a, b) increased the translocation of the inflammation-related transcription factors NF-κB p65 (a, c), c-Jun (a, d), and CEBPδ (a, e) to the cell nucleus. Data are expressed as the mean relative expression ± SEM of at least three independent experiments