Fig. 1

LPS regulates the expression of GRα and GRβ isoforms in U-2 OS transfected cells. a. Western blot of total proteins of parental U-OFF cells (line 1), U-2 OS alpha cells (line 2) and U-2 OS beta cells (line 3) are shown. On the left panel, a common antibody to GR was used and on the right panel a specific GRβ antibody was used. U2-OS alpha (b) and US-OS beta (c) cells were stimulated with LPS for 24 h. The expression of GRα and GRβ was determined by Western blot. The median values of three different experiments, plotted as values relative to control are shown. * p < 0.05 and ** p < 0.01