Fig. 1

3 T3 fibroblasts were grown in the presence and absence of GM-CSF and MSCs). GM-CSF increased the gene expression of collagen type I, III and HAs relative to the expression of these genes in 3 T3 cells at baseline without stimulation (p ≤ 0.05, n = 3). Using this modeling system of increased production of ECM related molecules we tested the capacity of bone marrow derived hMSC supernatants to alter the up-regulated ECM genes with GM-CSF. Treatment of the GM-CSF treated 3 T3 cells with hMSC supernatants decreased collagen type I, (1a) III (1b) and HAs gene expression (p ≤ .05, n = 3)