Fig. 2

Effect of 4-1BB deficiency on expression of mitochondrial oxidative metabolism-related genes/proteins in the skeletal muscle of HFD-fed obese mice a qRT-PCR analysis for markers specific to mitochondrial biogenesis/function (PGC-1α, COX8B, and NDUFA2) and fatty acid oxidation (PPARα, CPT1β, ACADM, and ACADL) in quadriceps muscles. b Immunohistochemical staining for COX IV in quadriceps muscle. Arrows indicate stained cells. Magnification × 200; Scale bars 50 μm. c-d PGC-1α and COX IV protein were measured by Western blotting. The relative intensities of the to α-tubulin control were measured using image J. e Phosphorylated AMPK (p-AMPK) and AMPK proteins were measured by Western blotting. The relative intensities of the bands of p-AMPK/AMPK were measured using image J. Data are means ± SEM; n = 6 in each group. *P < 0.05, **P < 0.01 when comparing the WT/HFD and KO/HFD groups