Fig. 4

Effect of oligomycin in macrophage phagocytosis and phenotype. Fresh elicited peritoneal macrophages were treated with different concentrations of oligomycin for 15 min. Phagocytosis was measured using the pHrodo S.aureus bioparticles kit and cell viability by AlamarBlue® assay. The expression of F4/80, CD16/32, and CD64 was evaluated by flow cytometry. a ECAR in control and oligomycin treated cells. b Phagocytosis of pHrodo S.aureus bioparticles in control and oligomycin treated macrophages. c Macrophage viability following oligomycin treatment. d Representative flow cytometry data showing the expression levels of F4/80, CD16/32 and CD64 in control and olygomycin treated macrophages. e The percentage of F4/80⁺, CD16/32⁺ and CD64⁺ cells in un-treated (UT) and oligomycin treated macrophages. Mean ± SEM, N = 4 mice. *p < 0.05, Student t test in a and One-way ANOVA, followed by Dunnett’s test in b