Fig. 3
From: MicroRNAs differentially expressed in Behçet disease are involved in interleukin-6 production
Upregulation of IL-6 transcript levels by transfection of microRNA inhibitors and mimics. THP-1 cells (2.5 × 104) were transfected with inhibitors for miR-638 and miR-4488 together with mimics for miR-3591-3p (miRNA) or with a mixture of control inhibitors and mimics (con). PAM-control microRNA (1/10) was cotransfected to determine the transfection efficiency. After 21 h, cells were stimulated with LPS (10 ng/mL) for 3 h and mRNA levels of TNF-α (a) and IL-6 (b) were assessed by qRT-PCR. Independent experiments were repeated more than three times in duplicate or quadruplicate. Relative TNF-α expression: mRNA level/average mRNA level of con in the absence of LPS. Relative IL-6 expression: mRNA level/average mRNA level of con in the presence of LPS. N.D.; not detected, **P < 0.005. Student’s t-test was used for the calculation of P values