Fig. 8

EPO and AG490 administrations increase the vessel density in quadriceps after critical limb ischemia induction in rats. The distribution of small vessels (diameter < 35 μm) was detected with antibodies against α-smooth muscle actin. a Quadricep section form normal rats. b Quadricep section form rats treated with critical limb ischemia (CLI). c Quadricep section form CLI rats treated with EPO. d Quadricep section form CLI rats treated with AG490. e Quadricep section form CLI rats treated with EPO and AG490. f Quantitation of number of small vessels in quadriceps. Yellow arrows indicate small vessels. Statistical analysis using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡, §) indicate significance at p value less than 0.05