Fig. 3

Representative in vivo ³¹P MR spectra from a the ipsilateral striatum of a 1 ng/μl IL-1β-injected animal, b the ipsilateral striatum of a 100 ng/μl IL-1β-injected animal and c the contralateral striatum of a 100 ng/μl IL-1β-injected animal. Each spectrum exhibits peaks from the phosphomonoesters (PME, a); inorganic phosphate (Pi, b); the phosphodiesters (PDE, c); phosphocreatine (PCr, d) and the three resonances of the gamma (γ, e), alpha (α, f) and beta (β, g) phosphates of adenosine triphosphate (ATP). d Representative ³¹P MR spectrum of the ipsilateral striatum of a saline-injected animal showing integration of the spectral peaks. (i) Original spectrum. (ii) Baseline correction and spectral fit using a software package compiling the AMARES algorithm [23, 24]. (iii) γATP and αATP are doublets and comprised of two resonances of equal area, γ: peaks 5 and 6, α: peaks 7 and 8 respectively. βATP is a triplet comprised of three resonances; two of which are of equal area: peaks 9,10 and 11. PCr (peak 1) is a singlet. (iv) The spectral residue (spectrum minus fit).