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Table 1 The pro-inflammatory combinations added to cultures of HCE cells and HCF

From: Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid

Combination 1

LPS(10 ng/ml) + IL-6+ YNFα + IFNγ + IL-1β

Combination 2

LPS(1 ng/ml) + IL-6 + TNFα

Combination 3

LPS(10 ng/ml) + IL-6 + TNFα

Combination 4

LPS(10 ng/ml) + TNFα + IFNγ

Combination 5

LPS(100 ng/ml) + IL-6 + TNFα + IFNγ + IL-1β

  1. The pro-inflammatory cytokines include: IFN- γ, TNF-α, IL-1β and IL-6. Each cytokines’ concentration was kept constant throughout all combinations, and was 100 ng/ml