Fig. 2
From: Nitric oxide secretion in human conjunctival fibroblasts is inhibited by alpha linolenic acid
Nitrite accumulation in stimulated cultured HCF cells with and without incubation with ALA. HCF cells incubated with ALA for 2 h and then exposed to 10 ng/ml LPS with cytokines, as described in combination 1. Nitrites were evaluated in supernatants after 72 h (n = 3). The asterisk (P < 0.01) represents statistical significance (P < 0.01) for stimulated HCF cells stimulated after treatment with ALA vs stimulated HCF cells without ALA. The double asterisk represents statistical significance (P < 0.01) for stimulated HCF cells vs HCF cells in medium alone. ALA - alpha-linoleic acid; HCF - human conjunctival fibroblasts