Fig. 7

C1q protein inhibits THP-1 macrophage inflammatory cytokine release. THP-1 macrophages were washed and resuspended in serum free medium (Xvivo 10 + L-glut) and pretreated with C1q (0–80 μg/ml) for 30 min before stimulation with LPS (100 ng/ml) for 24 h. Unstimulated controls were run in parallel. Supernatants were collected and TNF-α and IL-6 were measured by specific ELISA. Data shown is the average of n = 3–4 (IL-6 n = 3, TNF-α n = 4) independent experiments. * P value of <0.05, ** P value of <0.01, *** P value of <0.001