Fig. 5

Apoptotic AGS co-culture attenuates THP-1 macrophage production of inflammatory cytokines irrespective of the method of apoptosis induction. a AGS made apoptotic with camptothecin (CMPT; 3 μM) or with H. pylori (MOI 100) for 24 h were co-incubated with THP-1 macrophages for 2 h. Apoptotic AGS were washed off and the THP-1 macrophages were replaced with RPMI+ 10 % FBS + 10 mM HEPES + 100 ng/mL LPS. Supernatants were collected after 24 h of LPS stimulation and were stored at −80 °C until analyzed for IL-6 and TNF-α concentrations using specific ELISA. b shows that attenuation of LPS-stimulated (100 ng/mL) IL-6 release was independent of internalization as the presence of cytochalasin D (CytD; 5 μg/mL) treatment did not reverse the attenuation of IL-6 observed following CMPT or H. pylori apoptotic AGS co-culture. Data shown is the average of three-four independent experiments. * P value of < 0.05, ** P value of < 0.01