Fig. 4

Complement receptors are expressed on THP-1-derived macrophages and PBDM’s. a THP-1 cells or PBDM’s were incubated with antibodies to indicated C1q receptors or matched isotype controls for 30 min, washed, fixed, and analyzed by flow cytometry. b RNA from THP-1 cells or PBDM’s was isolated and mRNA for the indicated C1q receptors was amplified using Real-Time PCR. Values in the table represent mean critical threshold ± standard deviation. Data shown is the average of at least three independent experiments. c C1q binds to apoptotic AGS cells. AGS cells were treated with 3 μM camptothecin for 24 h, trypsinized, washed and then incubated in serum-free media (black) or serum-free + 40 μg/ml C1q (white) for 1 h. Cells were then incubated with a C1q-FITC antibody for 30 min and C1q binding was measured using flow cytometry. Data shown is the average of three independent experiments. * overall P value of <0.05