Fig. 2

Reconstitution of C1q restores binding of apoptotic AGS cells to THP-1 cells in the absence of serum. THP-1 cells were incubated for 1 h with H. pylori-infected (MOI 300) AGS cells in the presence of 10 % v/v non-heat inactivated fetal calf serum (black), in serum-free media (white), or serum-free + 40 μg/ml C1q (diagonal bars), after which time the slide was washed three times and the number of bound AGS cells was counted in four random fields. Data shown is the average of n = 5 independent experiments. * overall P value of < 0.05