Fig. 5

EGCG treatment increases IDO expression and activity in CD11b⁺ cells. CIA mice were fed with EGCG or PBS (vehicle) for 7 weeks after CIA induction and (a,b) dLN sections were stained for CD11b and IDO, respectively (magnification: X200). Photomicrographs and graph shown are representative of three independent experiments with 4 to 5 mice per group. (c,d) Expression of IDO in CD11b⁺ cells from dLN and spleen were analyzed by flow cytometry. Data are representative of three independent experiments with a total of 14 mice per group. (e) Functional IDO activity in freshly isolated CD11b⁺ cells was measured by the production of kynurenine in the culture supernatants of sorted DC as detailed in the methods. CD11b⁺ DC from spleens were sorted and incubated in HBSS for 4 h. Studies were performed in triplicate and repeated at least twice with similar results; in total seven mice were examined from each group