Fig. 4

EF24 reduces expression of phospho-NF-kB p65 in LPS-stimulated DCs. JAWS II DCs were stimulated with LPS (100 ng/ml) for 4 h, followed 1 h by treatment with 10 μM of EF24. (a) For confocal microscopy (63X objective), the nucleus was stained with Hoechst dye (blue), whereas NF-kB was stained (green) with antibody against the phosphorylated form NF-kB p65. A representative set of images from three separate experiments is shown. (b) The intensity of green fluorescence representing phospho-NF-kB p65 (mean ± sem) was quantified in at least five different fields per view. One way analysis of variance and Bonferroni post-test was performed on the data (# p value < 0.05 vs. control and * p value < 0.05 vs. LPS treatment). (c) The expression of phospho-p65 and total p65 in JAWS II DCs, evaluated by immunoblotting