Fig. 3

EF24 suppresses the LPS-induced IL-1R1 expression and IL-1β secretion in JAWS II DCs. (a) Quantitative-polymerase chain reaction for the measurement of the IL-1R1 mRNA expression (mean ± sem, fold changes as compared to control) in JAWS II DCs that were LPS (100 ng/ml)-stimulated for 4 h, followed by treatment with 10 μM EF24. # p value < 0.05 vs. control and * p value < 0.05 vs. LPS treatment. (b) Immunoblots of IL-1R1, IL-1R2, and IL-1RA expression in JAWS II DCs treated with EF24 and LPS. (c) IL-1β secreted in medium of cultured JAWS II DCs. The data is average (± sem) of samples from three separate experiments, each estimated in triplicate (# p value < 0.05 vs. control and * p value < 0.05 vs. LPS treatment)