Table 1 Primers used for the PCR detection of specific gene expression
From: Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
Gene | Forward | Reverse | PCR product size |
|---|---|---|---|
TGF-β1 | 5′-GACTCCTGCTGCTTT CTCC-3′ | 5′-GCGGTCCACCATTA GCAC-3′ | 531 bp |
IL-6 | 5′-CCACGGCCTTCCCTA CTTC-3′ | 5′-TTGGTCCTTAGCCAC TCCT-3′ | 499 bp |
COL1A1 | 5′-GTCCTATGGCTATGATG AGAA ATC-3′ | 5′-CACCATCCAAACCA CTGAAAC-3′ | 335 bp |
TNF-a | 5′-GTCCCCAAAGGGATGA GAAG T-3′ | 5′-TGAGATAGCCAAATCG GCT GAC-3′ | 323 bp |
GAPDH | 5′-ACCACAGTCCATGCCAT CAC-3′ | 5′-TCCACCACCCTGTTGCT GTA-3′ | 452 bp |