Figure 6

GW9662, like dexamethasone, inhibits in vitro production of TNF-α and IL-10. TNF-α (A and C) and IL-10 (B and D) production levels were quantified by ELISA in culture medium of cells incubated for 3 days in the presence or absence of GW9662 (2.5-10 μM) (n = 3) (A and B) or dexamethasone (1–100 nM) (n = 4) (C and D). When specified, LPS (50 ng/ml) was added in the last 48 hours. Data are represented as mean values ± SEM. Analysis was performed using one-way repeated measures ANOVA with Newman-Keuls multiple comparisons test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Comparison between spontaneous and LPS-triggered cytokine release was performed using Student’s paired t-test. When not specified by additional graphic signs, statistical annotations (asterisks) refer to comparisons respect to the relative GW9662 or dexamethasone untreated control. Each set of data is representative of three to four independent experiments.