Figure 2

M1 stimulation with IFN-γ or GM-CSF opposes GW9662 effects on macrophage phenotype. (A-B) Healthy monocytes were cultured in serum-free medium in the presence of IFN-γ (2.5 ng/ml) or GM-CSF (100 ng/ml; M1 differentiation), with or without the PPAR-γ antagonist GW9662 (10 μM), for 4 days. Expression of CD206, CD163 and MerTK was measured by flow cytometry. (B) Pooled data are represented as mean values ± SEM. Analysis was performed using Student’s paired t-test. *P < 0.05; **P < 0.01; n.s., not significant. Data are representative of three independent experiments.