Figure 7
The effect of GPX and curcumin on IL-8 secretion in Sc and Gi HepG2 cells with or without palmitate treatment. (a) The IL-8 secretion of Sc and Gi HepG2 cells co-treated with 2 U/ml glutathione peroxidase (GPX) and 0.6 mM palmitate (PA) for 24 hr was measured by ELISA. These results are representative of at least three separate experiments. *indicates a significant difference (P < 0.05) between Sc and Gi HepG2 cells. α indicates a significant difference (P < 0.05) between PA without GPX and PA-GPX co-treated HepG2 cells. (b) The IL-8 mRNA of Sc and Gi HepG2 cells co-treated with 50 or 100 μΜ curcumin (referred to cur50 and cur100, respectively) and 0.6 mM palmitate (PA) for 6 hr was measured by qRT-PCR. These results are representative of at least three separate experiments. *indicates a significant difference (P < 0.05) between Sc and Gi HepG2 cells. #indicates a significant difference between control and curcumin-treated HepG2 cells without palmitate. α indicates a significant difference (P < 0.05) between PA without curcumin and PA-curcumin co-treated HepG2 cells.