Figure 4

The effect of NF-κB inhibition on IL-8 secretion in HepG2 cells. (a) The effect of NF-κB inhibitor on IL-8 secretion with or without palmitate treatment. The IL-8 secretion of Sc and Gi HepG2 cells treated with 10 μM NF-κB inhibitor (BAY11-7085) with or without 0.6 mM palmitate for 24 hr was measured by ELISA. These results are representative of at least three separate experiments. *indicates a significant difference (P < 0.05) between Sc and Gi HepG2 cells. ^#indicates a significant difference (P < 0.05) between control and inhibitor (BAY)-treated HepG2 cells without palmitate. α indicates a significant difference (P < 0.05) between palmitate (PA)-treated cells without inhibitor (BAY) and palmitate (PA)-inhibitor (BAY) co-treated HepG2 cells. (b) The effect of p65 knockdown in Sc and Gi HepG2 cells on p65 protein level determined by Western blotting. The blot (cell lysate: 25 μg protein content) was incubated with anti-p65 antibody and subsequently stripped for incubation with anti-actin antibody as loading control. (c) The effect of p65 knockdown on IL-8 gene expression by qRT-PCR and (d) IL-8 secretion by ELISA. These results are representative of at least three separate experiments. *indicates a significant difference (P < 0.05) between SiRNA- control and SiRNA- p65.