Table 1 Primer sequences used in the study
Gene | 5′-3′ primer sequence | Product size (bp) | GenBank accession |
|---|---|---|---|
Shh | Forward: TCCGATGTGTTCCGTTACC | 100 | NM_017221.1 |
Reverse: AACCTTGCCTGCTGTTGC | |||
Ptch1 | Forward: CACCAAGTGATTGTGGAAGC | 102 | NM_053566.1 |
Reverse: CTGTTGCCGAGAGTTCAAGG | |||
Smo | Forward: ATGCGTGTTTCTTTGTGGGC | 133 | NM_012807.1 |
Reverse: ACACAGGATAGGGTCTCGCT | |||
Gli1 | Forward: AACTCCACGAGCACACAGG | 106 | NM_001191910.1 |
Reverse: GGCAGTCCGTCTCATACACA | |||
COII | Forward: TCAAGTCGCTGAACAACCAG | 116 | NM_012929.1 |
Reverse: GTCTCCGCTCTTCCACTCTG | |||
aggrecan | Forward: GCAGCACAGACACTTCAGGA | 137 | NM_022190.1 |
Reverse: CCCACTTTCTACAGGCAAGC | |||
β-actin | Forward: TTGCTGACAGGATGCAGAA | 101 | NM_031144.3 |
Reverse: ACCAATCCACACAGAGTACTT |