Figure 4

Effect of cytokines on gene expression of Il6 (A) and Tnfα (B) in Caco2 cells. Differentiated Caco2 cells were pre-treated with or without r-h-TNF-α (20 ng/ml), r-h-IL-6 (20 ng/ml) or their combination for 72 h before changing into the medium with or without LA (1 mM) for 24 h. The mRNA levels of both Il6 and Tnfα were measured by real time RT-PCR. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle control; and ^# P <0.05, ^### P <0.001 vs. LA.