Figure 3
LA stimulated gene expression of Il6 and Tnfα in Caco2 cells. Differentiated Caco2 cells were supplemented with or without 1 mM LA for the indicated times for mRNA measurement or with different amount of LA for 24 h for protein measurement. The mRNA levels of Il6 and Tnfα were measured by real time RT-PCR (A and B). Their proteins from culture medium were measured by ELISA (C and D). *P < 0.05, ** P <0.01, *** P <0.001 vs. vehicle control.