Figure 4

Effect of Ara 3000 beta® on NO and PGE2 release, and NFκB DNA binding. Chondrocytes were pre-incubated 24 hours with Ara 3000 beta® or vehicle, and then they were treated with IL-1β (IL1) or vehicle (C), in the presence or not of Ara 3000 beta® for 24 h. A, B- At the end of treatment, mediums were collected, and NO and PGE2 levels were determined in medium by Griess assay (A) and ELISA (B), respectively. Histograms represent the percentage of induction of NO or PGE2 in Ara 3000 beta® treated-cells normalized to cells treated with IL-1 without Ara 3000 beta®. C- Electrophoretic mobility shift assay (EMSA) were performed using NFkB consensus binding probe, incubated with nuclear extracts from chondrocytes treated as previously. Arrow indicates the band corresponding to NFkB binding.