Figure 1
From: Knockdown of MVK does not lead to changes in NALP3 expression or activation
Analysis of inflammasome components expression after siRNA treatment (a) ΔΔCt analysis of mRNA expression. Left MVK expression, right NALP3 expression. mRNA expression in cells trasfected with “scrambled” sequences were considered as reference. * = p < 0.05 in comparison with UNT, i.e. untreated condition, cells subjected to transfection but without any siRNA (b) Representative western blot: UNT cells subjected to transfection but without any siRNA. (c) Optical density calculation of MVK protein levels. Scrambled (SCR) condition was set as 1. * = p < 0.05(d) Optical density calculation of NALP3 expression.