Figure 6
Examination of the type of AR involved in the anti-inflammatory activity of ANO. Sodium butyrate-treated THP-1 cells (1 × 105 /well) were pretreated with 2 μM of A1 AR-, A2A AR-, A2B AR- and A3 AR-selective antagonists (DPCPX, ZM241385, MRS1754 and MRS1911, respectively) for 30 min before stimulation with LPS plus huIFN-γ in the presence of 10 μM ANO. After 24 h, levels of TNF-α in the culture supernatants were determined by ELISA. Values represent the means ± S.D. of triplicate cultures. Results are representative of two separate experiments with similar results. ##, p < 0.01, significantly different when compared with vehicle control culture in the presence of ANO.