Figure 5

Hdac1 depletion does not modify JQ1 inhibitory action on IL-1β-dependent Ccl2, Ccl5 and Cxcl2 mRNA expression in IEC-6 cells. ShCtrl and shHDAC1 IEC-6 cells were pre-incubated for 4 h with 1 μM of the JQ1 bromodomain acetyl-binding inhibitor, before adding 10 ng/ml of IL-1β for an additional 8 h. Total RNAs were isolated and expression levels of various inflammatory response genes (Ccl2, Ccl5, Cxcl2 and Cxcl12) were assessed by semi-quantitative RT-PCR. ShCtrl and shHDAC1 cDNA fragments for Ccl2, Ccl5, Cxcl2, Cxcl12 and Gapdh were respectively separated by electrophoresis on the same gel, and were detected by ethidium bromide staining. Gapdh was used as a control for the amount of cDNA amplified. Results are representative of three independent experiments.