Figure 4

Hdac1 depletion differentially modulates inflammatory protein secretion in IEC-6 cells. Two shCtrl and shHDAC1 IEC-6 cells were treated with or without 10 ng/ml of IL-1β for 24 h. Supernatants from untreated or IL-1β treated shCtrl and shHDAC1 cells were harvested and cytokine secretion was measured with a commercial cytokine and chemokine array. The histograms represent the mean fluorescence intensity of duplicate experiments, measured with a ScanArray Express Microarray scanner. Secreted protein expression patterns are shown in A (Cxcl3, VEGF, Ccl2), B (Ccl20, CNTF), C (Cx3cl1, Timp1, Cxcl2), and D (Cxcl5, β-NGF).