Figure 5

LPS stimulated upregulation of NF-ĸB in VFF . Scar and polyp vocal fold fibroblasts (VFF) stimulated with lipopolysaccharide (LPS) for 45 minutes had higher percentage of NF-κB activation as detected by immunocytochemistry. NF-κB p65 translocation into the nucleus was determined by immunofluorescence. The first column denotes normal (T21 & T59), scar, and polyp VFF baseline percentage levels without LPS and second column shows levels after LPS stimulation.