Figure 3

Comparison of normal, scar, and polyp VFF gene expression after LPS stimulation. Cells were treated with 5 μg/mL LPS for 24 hrs. RNA was subsequently extracted, converted into cDNA, and then analyzed for (A) TLR4 and (B) IL-1 receptor 1 gene expression using RT-PCR. Amplification efficiencies of target and reference genes were assessed by LightCycler software. Standard curves were derived to quantify gene expression and concentrations were normalized to β-actin housekeeping gene. Letters represent a statistical significance of p < 0.05 when compared to (a) T21 (b) T59 (c) scar (d) polyp. * represents differences within cell type with (w/) LPS compared to without (w/o) LPS.