Figure 5
From: Positive allosteric modulation of the adenosine A2a receptor attenuates inflammation
Dose-dependent inhibition of LPS- and PMA- stimulated TNF-α production upon allosteric potentiation of the A 2A R. Human monocytes were stimulated with LPS (5 ng/ml; A) or phorbol 12-myristate 13-acetate (PMA; 5 ng/ml; C) for 12 h whereas mouse monocytes were stimulated with LPS (50 ng/ml; B) for 18 h in the presence of varying concentrations of AEA061. TNF-α in the culture medium was quantified as described in Materials and Methods. Values and error bars represent means and SEMs of one of three representative experiments performed in triplicate.