Figure 1

Potentiation of agonist-mediated A₂R activation in PC-12 cells. (A) AEA061 enhances adenosine-mediated cAMP production. PC-12 cells were incubated for 30 min with adenosine and AEA061. EC₅₀ values in μM (with 95% confidence intervals in parentheses) were as follows: control = 9.7 (7.8 – 12.0); AEA061 (1 μM) = 9.5 (7.5 – 12.2); AEA061 (10 μM) = 0.84 (0.3 – 2.4); AEA061 (100 μM) = 0.83 (0.74 – 0.94). (B) AEA061-mediated potentiation of cAMP production is sensitive to ZM241385. PC-12 cells were pre-incubated with ZM241385 (ZM) and MRS 1754 (MRS) for 15 min prior to treatment with adenosine (Ado; 1 μM) and AEA061 (1 μM) for additional 30 min. (C) AEA061 enhances cAMP production mediated by CGS 21680. PC-12 cells were incubated for 15 min with AEA061 and CGS 21680. Mean cAMP levels ± SD of a representative experiment in triplicates are shown.