Acute tubular necrosis (ATN) following ischaemia was unaffected following AC administration. Renal IRI was induced in 8-week old male Balb/c mice by a right nephrectomy and ischaemia induced by occluding the left renal pedicle for 20 (mild ischaemia), 24 (moderate) or 25 mins (severe). Sham mice underwent a laparotomy only. Either PBS or 20×106 early or late ACs were administered 24 hr prior to renal IRI. Mice were sacrificed 24 hr following IRI. (A) Representative images of the outer stripe of the outer medulla (OSOM) in non-injured and ischaemic kidney sections stained with H&E. Tubules within the OSOM were classified as necrotic (black arrow) or healthy (grey arrow) according to cell morphology and integrity. (Magnification: ×200; Scale Bar: 50 μM) (B) Scoring of ATN (expressed as the percentage of necrotic tubules) demonstrates that the structural injury remained similar between PBS and AC treated mice with severe, moderate and mild ischaemic injury. Grey circle symbol = Non-injured kidney Grey square symbol = Ischaemic kidney. Data expressed as mean ± SEM and analysed by two-way ANOVA. ns = non-significant. Sham (n = 4), PBS (n = 7–8), AC (n = 8).