Figure 4

FACS analysis of various immune cell sub-types in the lung and BALF. The absolute numbers of various immune cell subtypes in the lung were counted (A, B) (described in Materials and methods). The lung and BALF cells were stained with FITC-conjugated mAb to CD3 and CD11b as well as PE-conjugated mAb to Gr-1 and CCR3. A dot-plot pattern is shown for the representative flow cytometric profile for the gated CD3-/CCR3+ and Gr-1+/CD11b + cells (C, D). The results are expressed as the mean ± SEM (n = 5). The statistical significance of differences between the control and treatment groups was assessed by ANOVA or the nonparametric Mann-Whitney test followed by Dunnett's multiple comparison test (*P < 0.05, **P < 0.01, ***P < 0.001). N: Normal BALB/c mice, CT: Ovalbumin inhalation + vehicle, OVA + CF (50, 200 mg/kg).